Basic Bioinformatics Tools Workshops: Differential Expression Analysis of RNAseq Data

Instructor: Tristan De Buysscher, Bioinformatics Scientist
Location: HSL 307

RNAseq provides a snapshot of the transcription state of the cells from which RNA was harvested. One of the most common applications of this sequencing data is to look at changes in the transcriptome state between two or more conditions the cells were exposed to. We will discuss the various considerations in analyzing RNAseq data and run through a practical example using DESeq2.

Account Requirement: You will need an account on the “Longleaf” Linux cluster managed by ITS-Research Computing. Instructions for requesting a “Longleaf” account via the ONYEN Subscribe to Services utility is available at this link: http://help.unc.edu/help/how-do-i-get-an-account-on-killdevil-research-computing-cluster/. Please submit your account request at least 3 days before class. It generally takes 24-48 hours for account creation.

Prerequisites: For those unfamiliar with UNIX and Longleaf/UNC computer cluster usage, UNIX for Biologists, Part 1 and UNIX for Biologists, Part 2 (from this series or prior offerings) are prerequisites for this class.