Basic Bioinformatics Tools Workshops: BBTools: A Toolbox for High Throughput Sequencing Data Analysis

Instructor: Tristan De Buysscher, Bioinformatics Scientist, UNC Center for Bioinformatics
Location: HSL 307

The BBTools suite contains tools that assist with multiple types of high throughput (HT) -sequencing data analysis. After an overview of tools included in BBTools toolbox, we will learn how to use “bbduk” (decontamination using k-mers) for scanning/trimming data to remove adapter contamination. This will be followed by “bbmap” (sequence data aligner) which we are going to use to align the test sequence data.

Account Requirement: You will need an account on the “Longleaf” Linux cluster managed by ITS-Research Computing. Instructions for requesting a “Longleaf” account via the ONYEN Subscribe to Services utility is available at this link: http://help.unc.edu/help/how-do-i-get-an-account-on-killdevil-research-computing-cluster/. Please submit your account request at least 3 days before class. It generally takes 24-48 hours for account creation.

Prerequisites: For those unfamiliar with UNIX and Killdevil/UNC computer cluster usage, UNIX for Biologists, Part 1 and UNIX for Biologists, Part 2 (from this series or prior offerings) are prerequisites for this class.